The data on net closures shows four regions — Tower Hamlets, Hackney, Southwark and Newham — have lost one third of their pubs since 1997.
And two areas have experienced a three-figure reduction: Southwark (110) and Liverpool (100).
The figures, obtained via a Parliamentary question, are based on data from the Valuation Office Agency.
They compare the number of pubs per local authority area in 1997 with 2009 and show an overall fall from 49,780 to 46,250.
Shadow local government minister Bob Neill said: “Under Labour there has been a surge in alcohol-fuelled violence in our high streets, while local community pubs have gone to the wall.
So with each closure the people of the local community are deprived of somewhere to meet, local groups who often meet in pub rooms are pushed out, whilst the staff often end up out of work. One other factor is the amount of money raised for charity by local pubs, an often forgotten fact is that many thousands of pounds are generated through donations by regulars to charities.
Each time a pub closes the cost to the community is more than just an empty building with a for sale sign on it.
Any drinker who votes Labour is calling for more pub closures, more duties and regulations of their lifestyle choice and helping to destroy the pub trade.